Brief introduction: In the research of DNA vaccine and gene therapy in the world, one of the most important technical difficulties is how to improve the efficiency of gene transfer into animal living cells. The key to solve this problem is to research and develop an instrument that can transfer exogenous DNA safely and efficiently into tissue cells. Using specific electric field to make living cells in pulsed electric field can significantly enhance the permeability of cell membrane, so that DNA molecules can enter the cells in large quantities, and improve the effect of DNA vaccine and gene therapy. The instrument has the same characteristics of high efficiency, simplicity and safety after being compared with similar instruments abroad. Areas of application: In vivo gene transfer apparatus plays an important role in nucleic acid vaccine, gene therapy, antibody preparation, animal transgenic, in vivo gene function verification siRNAs, recombinant subunit vaccine, gene function test and other transgenic research. Characteristics: 1. Efficient, fast, safe 2. Microelectronics and specific control circuits 3. Target organ is unlimited 4. There is no restriction on the size of foreign gene fragment 5. Simple and fast operation 6. Only a few seconds. 7. DNA fragment does not require special purification operation Technical parameters: Input voltage: 220V AC/50Hz or 60Hz Power: 50W Pulse voltage: 10 / 200 VDC / 1 VDC step) Pulse width: 1-255 MS (1ms step) Gap width: 10-999MS(10ms step) Number of pulses: 1-50 Random electrode: one needle electrode Display mode: digital display.
Brief introduction: In the research of DNA vaccine and gene therapy in the world, one of the most important technical difficulties is how to improve the efficiency of gene transfer into animal living cells. The key to solve this problem is to research and develop an instrument that can transfer exogenous DNA safely and efficiently into tissue cells. Using specific electric field to make living cells in pulsed electric field can significantly enhance the permeability of cell membrane, so that DNA molecules can enter the cells in large quantities, and improve the effect of DNA vaccine and gene therapy. The instrument has the same characteristics of high efficiency, simplicity and safety after being compared with similar instruments abroad. Areas of application: In vivo gene transfer apparatus plays an important role in nucleic acid vaccine, gene therapy, antibody preparation, animal transgenic, in vivo gene function verification siRNAs, recombinant subunit vaccine, gene function test and other transgenic research.
