Introduction: The portable gene gun is a portable gene gun device that can directly transfer cells in situ and is suitable for transient and stable transfection. The instrument has a small shooting area and uniform particle distribution, and can transform receptors of different sizes. , To ensure transformation efficiency; low sample requirements: only a small amount of DNA and cells, no carrier DNA, no restriction on large fragments of DNA. Hand-held gene guns are widely used in gene transformation studies of animal and plant samples due to their simple and flexible operation. The SJ-500 portable gene gun has a special mechanism that generates gas shock waves, and the generated air pressure rating range: 0.5Mpa-5Mpa. Using helium or nitrogen as the power source, the SJ-500 portable gene gun produces a kind of "cold" gas shock wave carrying DNA microprojectiles into the receptor, so it can avoid cell damage caused by the "hot" gas shock wave, high quality To complete the genetic modification work. Features: 1. Strong targeting, will not cause irrelevant genes or proteins to be introduced into the sample 2. Convenient and fast, portable and portable design, can carry out transformation work on site 3. Strong functionality Compared with other methods, it can achieve cell transformation with special growth needs 4. Low sample requirements, only a small amount of DNA and cells, no carrier DNA, no restriction on large fragments of DNA 5. High conversion rate, small shooting area and uniform particle distribution, which can transform receptors of different sizes, ensuring the conversion efficiency Technical Parameters: Experimental conditions: in situ, in vitro, in vivo Target area: (small) 2cm² Pressure range: 0.5-5MPa (12 times per revolution of the magazine) Target type: animal cells, plant cells, living plant leaves, Living animal skin, muscle, living animal organ Voltage: 9V (Can launch 1000 times) Gas source: helium or nitrogen Metal particles: Φ0.8-1.5μm; φ1.5-3.0μm Bombardment method: direct sampling or nylon tube preparation
Introduction: The portable gene gun is a portable gene gun device that can directly transfer cells in situ and is suitable for transient and stable transfection. The instrument has a small shooting area and uniform particle distribution, and can transform receptors of different sizes. , To ensure transformation efficiency; low sample requirements: only a small amount of DNA and cells, no carrier DNA, no restriction on large fragments of DNA. Hand-held gene guns are widely used in gene transformation studies of animal and plant samples due to their simple and flexible operation. The SJ-500 portable gene gun has a special mechanism that generates gas shock waves, and the generated air pressure rating range: 0.5Mpa-5Mpa. Using helium or nitrogen as the power source, the SJ-500 portable gene gun produces a kind of "cold" gas shock wave carrying DNA microprojectiles into the receptor, so it can avoid cell damage caused by the "hot" gas shock wave, high quality To complete the genetic modification work.
