Introduction RunGel real-time detection electrophoresis was applied in onventional nucleic acid gel electrophoresis separation;Suitable for realtime gel electrophoresis;Suitable for rapid DNA fragment scanning and screening;Suitable for quick and easy nucleic acid fragment recycling. Features High resolution, real-time observation of the nucleic acid gel electrophoresis process;
High recovery rate, can direct recovery of DNA fragments, omit artificial cut rubber and rubber recycling purification process;
Only fragments by ethanol precipitation can be recovery in the downstream test;
Blue LED light source to replace the traditional uv light, safe environmental protection, no damage to the human body;
Wide application, can be used to observe dyeing such as recycling, EB, SYBR SAFE nucleic acid samples. Technical parameters (A) observation device:
1. Operating modes: constant voltage or constant current.
2. Voltage range: 25-150 V;
3. The voltage resolution: 1 v.
4. Current: 300 mA;
5. The current resolution: 1 mA;
6. Power is: 45 W;
7. Blue LED excitation light source: 470 nm;
8. The timer: 1-999 minutes, there is alarm;
9. Operating temperature: room temperature 40 ℃;
10. Size: 293 * 220 * 80 mm;
11. Working voltage: 100-240 v, 50/60 hz.
(B) electrophoresis device:
1. Observe the cover design: orange spectral filter used in the dyeing samples such as observation SAFE or red spectral filter is used to observe the EB dyeing samples;
2. Gel size (W * L) : 12 *12, 12 * 6, 6 * 12, 6 * 6 cm;
3. The electrophoresis tank size (W * D * H) : 26.5 * 17.5 * 9 cm;
4. The buffer capacity: 500 ml.
Four, product configuration:
RunGel system (equipped with orange spectral filter), 12 x 12 cm plastic tray, 12 x 6 cm plastic tray, 6 x 12 cm plastic tray, 6 x 6 cm plastic plate and comb 1 set.
Five, the fragments of nucleic acid recovery process:
1. The system of glue: use a standard preparation electrophoresis of tooth comb to make 2 lines matching hole, a row of holes for "the hole", another hole to hole "recycling";
2. Transfer through the blue rubber sheet to electrophoresis groove, the electrophoresis tank on the base;
3. Add enough buffer to cover gel, remove tooth comb electrophoresis, the DNA sample on to the top of the sample holes;
4. Cover the electrophoresis tank lid, connect the power cord, switch on, open the blue light transmission light source;
5. By real-time observation of nucleic acid electrophoresis tank cover migration;
6. When nucleic acid stripe migration into the recycling hole, turn off the power supply, open the electrophoresis tank cover, the recovery of DNA fragments by pipetting gun directly.
Introduction
RunGel real-time detection electrophoresis was applied in onventional nucleic acid
gel electrophoresis separation;Suitable for realtime gel electrophoresis;Suitable for rapid DNA fragment scanning and screening;Suitable for quick and easy nucleic acid fragment recycling.
