Introduction: The whole set of high temperature and high pressure disinfection and sterilization refers to the pipette made of materials resistant to high temperature of 121 ℃, the whole pipe is placed in an autoclave for 121 ℃/20min high temperature sterilization, completely eliminating the residue in the pipette suction rod and The cross-infection and spread of various deadly viruses and bacteria such as AIDS (AIDS), SARS (SARS), avian influenza, hand-foot-mouth disease, plague, mad cow disease, etc. in the heart, in order to protect the personal safety of users in the long term. Pipettes that are not resistant to high temperature sterilization will have a large amount of virus and bacteria inside and outside; half of high temperature sterilized pipettes (only the disassembled pipette rod) cannot be removed in the form of water vapor that crosses the pipette rod and penetrates the inside of the handle. Viruses will leave a chance for the virus to spread. The entire 121 ℃ high temperature sterilization pipette can withstand high pressure sterilization, so that users have no worries. It is also suitable for high temperature and high pressure disinfection of non-lethal but also infectious bacteria and viruses.