Instroment "Visualization" technology and related equipment development more and more pay the attention in clinical diagnostic tests and pathological studies of the related fields in life science research. Electrochemical luminescence imaging technology is a kind of imaging technology based on the electrochemical luminescence response, and it not only can provide information on electrochemical signals and luminescence intensity, but also can analysis the electrochemical optical imaging. As a new biochemical analysis method, electrochemical luminescence imaging device have the characteristic of simple, reaction control, informationization, visualization, high sensitivity and high-flux. Compared with the fluorescence imaging method, it has without light bleaching, light source and spectral system; compared with the chemiluminescence imaging method, it have the advantage of strong controllability; Relative to the electrochemical detection, it have the advantages of low detection limit and less of the electrode pollution. Due to the electrochemical luminescence, markers are fixed on the electrode surface in biological sensing imaging, so there is no diffusion problem to affect the resolution of imaging quantity. The instrument is high quantum yield, short response time, good stability, and it can be used for high sensitive electrochemical luminescence imaging analysis. Our company research and development of REFP-1 electrochemical luminescence imaging analyzer used EMCCD image sensor, the requirement of environment is low, operation simple and convenient, camera time is short, high sensitivity, and it can obtain multiple images and stored. In order to further improve the detection sensitivity, instrument designed PMT detecting system, which can interchange with the location of imaging system, so it can finish higher sensitivity testing requirements. Based on the electrochemical luminescence, high sensitive, reaction control, fast imaging and the analysis for biological compatibility, the instrument can be applied to potential fingerprint appeared, composition identification, DNA testing, doping tests, clinical diagnosis, explosives detection and other fields, it has important medical diagnosis, pathological study and security value. In biology aspect, it can be applied to the immune analysis, gene expression, tissue section and single cell test, in vitro organ perfusion, animal testing, cancer research, cell transplantation, infectious disease, apoptosis, interaction between protein and protein. It integrated of imaging analysis, biological analysis and fingerprint identification, and it can provide a new method and train of thought for the electrochemical luminescence imaging technology in life science and the application of forensic science. Composition REFP-1 electrochemical luminescence imaging analyzer consists of detection system and the control system: * Test system mainly consists of PMT components, EMCCD imaging detection components, precision mobile platforms. Samples can be measured accurately moved to the corresponding test area, respectively, for detecting imaging and lighting. * Control system mainly includes electrochemical measurement, luminescence measurement, mobile platforms and multiple electrode potential control, and it accept from computer system to control instruction, to control precise position of the detected PMT and EMCCD imaging, and to make sure entire system of data acquisition, image display. Application field: Potential fingerprint appeared, composition identification DNA testing, doping tests, clinical diagnosis Mineral substance and explosives detection Immune analysis, gene expression, tissue section and single cell test YOrgan perfusion, animal testing, cancer research, cell transplantation YInfectious disease, apoptosis, interaction between protein and protein Technical parameters: 1. Electrochemical analysis: Potential scope: - 10 V ~ 10 V Current range: ±250 mA Sensitivity: 1 × 10-9 ~ 1 × 10-2 A Scan rate: 0.0001 ~ 200 V/S 2. Light detection system: Measurement dynamic range: more than five orders of magnitude Accuracy of measurement: better than 0.05% Amplifier output drift: better than 0.05% Signal to noise: 0.5 mV (P - P values, 1×) Integral amplifier integral time: 0.001 ~ 10 s The system automatically zero Sampling rate: 1 ~ 200 times per second Response wavelength: 230 ~ 920 nm 3. EMCCD (which can be chosen by the user) Resolution: 512 (H) × 512 (V) pixels Photosensitive size: 8.192 mm (H) ×8.192 mm (V) Read rate: 417 or less frame/s frame Dark current: 0.01 electron/pix/s Testing light emitting area: 5 mm 5 mm Optical magnification: 40 ×, 20×, 10 ×, 4 ×
Instroment "Visualization" technology and related equipment development more and more pay the attention in clinical diagnostic tests and pathological studies of the related fields in life science research. Electrochemical luminescence imaging technology is a kind of imaging technology based on the electrochemical luminescence response, and it not only can provide information on electrochemical signals and luminescence intensity, but also can analysis the electrochemical optical imaging. As a new biochemical analysis method, electrochemical luminescence imaging device have the characteristic of simple, reaction control, informationization, visualization, high sensitivity and high-flux. Compared with the fluorescence imaging method, it has without light bleaching, light source and spectral system; compared with the chemiluminescence imaging method, it have the advantage of strong controllability; Relative to the electrochemical detection, it have the advantages of low detection limit and less of the electrode pollution. Due to the electrochemical luminescence, markers are fixed on the electrode surface in biological sensing imaging, so there is no diffusion problem to affect the resolution of imaging quantity. The instrument is high quantum yield, short response time, good stability, and it can be used for high sensitive electrochemical luminescence imaging analysis. Our company research and development of REFP-1 electrochemical luminescence imaging analyzer used EMCCD image sensor, the requirement of environment is low, operation simple and convenient, camera time is short, high sensitivity, and it can obtain multiple images and stored. In order to further improve the detection sensitivity, instrument designed PMT detecting system, which can interchange with the location of imaging system, so it can finish higher sensitivity testing requirements. Based on the electrochemical luminescence, high sensitive, reaction control, fast imaging and the analysis for biological compatibility, the instrument can be applied to potential fingerprint appeared, composition identification, DNA testing, doping tests, clinical diagnosis, explosives detection and other fields, it has important medical diagnosis, pathological study and security value. In biology aspect, it can be applied to the immune analysis, gene expression, tissue section and single cell test, in vitro organ perfusion, animal testing, cancer research, cell transplantation, infectious disease, apoptosis, interaction between protein and protein. It integrated of imaging analysis, biological analysis and fingerprint identification, and it can provide a new method and train of thought for the electrochemical luminescence imaging technology in life science and the application of forensic science.
