Pilot200 preparative liquid chromatography Introduction
The pilot200 preparative liquid chromatograph is a highly efficient and powerful modular preparative chromatography system with a small volume and large throughput, which improves the purification process of Chinese herbal active ingredients, chemical synthesis and biochemical drug separation, allowing up to 4 Gradient elution of different solvents, simultaneous on-line detection of two wavelengths, easy to store and call methods, and complete fraction collection on the same platform, support system connection of stainless steel column and high pressure glass column, widely used The separation, purification and preparation of target active ingredients such as Chinese herbal medicines, natural products, organic synthetic products and protein biopolymers can meet their requirements from laboratory research to industrial production. Features
Rapid purification and preparation of small molecule and macromolecular active ingredients
Highly flexible modular configuration with integrated fraction collection for a more intuitive scientific process
Cam curve compensation technology effectively controls flow pulsation to ensure minimum baseline noise
Multi-point flow calibration curve to ensure flow accuracy and system repeatability over the full flow range
The pump head has an automatic cleaning function to avoid buffer residue and crystal precipitation.
Simultaneous dual-wavelength detection in the 190-800nm range for reliable online analysis of major components, by-products and impurities
Easy replacement of lamps and flow cells, intelligent switching of tungsten lamps to ensure maximum running time
High-sensitivity online detection, the system supports a variety of detectors such as UV, ELSD, RI, etc.
Mixer flow path optimization for best elution mixing efficiency and minimum dead volume
316L stainless steel, PEEK, pure titanium, Hastelloy, a variety of materials for the flow path
Fully automatic fraction collector with multiple collection modes for configurable collection tubes
Project management uses database mode to manage data. Instrument methods, spectral data, and analysis methods associated with the project are all under the project.
Strict rights management, electronic records, electronic signatures, audit trails, user registration and multiple permission settings
System control software is fully compliant with CFDA GxP and FDA 21CFR Part 11 regulations
The system can be connected to any brand of column, SPE solid phase extraction column Technical Parameters
Pump head: 316L stainless steel pump High precision, low pulse, corrosion resistant (peek pump head optional)
Flow rate range: 0.01-200.00ml/min (gradient)
Flow rate accuracy: ±0.5%
Pressure range: 0-10MPa
Pressure pulsation: ≤0.2MPa
Gradient type: step, linear gradient, online gradient and flow rate
Minimum gradient adjustment: 1%
Detector light source: xenon lamp + tungsten lamp (import)
Detection wavelength: 190-800nm full wavelength detector dual wavelength simultaneous detection
Wavelength accuracy: ±1nm
Absorbance range: 0-2AU
Collection: Fully automatic collector
Collection pipe rack: 2×60 test tubes (Φ15mm*150mm test tubes) Other specifications can be selected
Collection mode: Normal mode (collected by time, peak collection, threshold collection), sequential collection, cyclic collection
Manual loading valve: Prepare the chromatographic valve (standard 10ml loop)
Loading method: Solid loading or liquid loading
Power supply: 220V±10% 50Hz
Chromatography software control: Controls pump, UV, automatic collector and other component setup and operation with sanochrom chromatography software
Control interface: Graphic interface, USB interface + RS-232 interface, PC software workstation based on Windows7/Windows 8/Windows 10, software meets the requirements of "CFDA GXP and FDA 21CFR Part 11"
Introduction
The pilot200 preparative liquid chromatograph is a highly efficient and powerful modular preparative chromatography system with a small volume and large throughput, which improves the purification process of Chinese herbal active ingredients, chemical synthesis and biochemical drug separation, allowing up to 4 Gradient elution of different solvents, simultaneous on-line detection of two wavelengths, easy to store and call methods, and complete fraction collection on the same platform, support system connection of stainless steel column and high pressure glass column, widely used The separation, purification and preparation of target active ingredients such as Chinese herbal medicines, natural products, organic synthetic products and protein biopolymers can meet their requirements from laboratory research to industrial production.
