Introduction: Cell compartments are commonly used in cell experiments, such as co-culture experiments, chemotaxis experiments, cell migration experiments, cell invasion, and drug delivery. Among them, permeability supports can effectively improve the culture of polar cells, because these supports allow cells to secrete and absorb molecules from their basal and top surfaces, thereby metabolizing in a more natural way, and mimicking the internal environment to the greatest extent To cultivate some special cell lines. Polycarbonate (PC) The high density of membrane pores allows more exchange through the membrane, and has excellent cell adhesion characteristics, so it is suitable for transport research and other applications that require optimal cell growth. In addition, PC film is also very suitable for barrier analysis. Compared with other membrane materials, it has a higher, more sustained and more stable transepithelial resistance (TEER) value over a longer period of time. PC membrane can culture most types of cells without matrix coating. PET film The PET film has a lower hole density and better optical clarity, which is conducive to microscopic cell observation and cell imaging.