HiCC-S1 automatic colony counting and inhibition zone measuring instrument
Technical Parameters: 1. Imaging device a It is equipped with a solid-state hard drive computer and an autofocus large depth of field 8 million pixels (3264x2448 pixels) color shooting light box capable of macro shooting. The highest resolution is 0.02mm, which can identify colonies as small as 0.05mm. With A4 format color CCD scanning imager (adjustable brightness, auto focus), it can realize floating dark-field imaging with an optical resolution of 40 million pixels b Fully enclosed dark box to eliminate environmental stray light interference c The upper and lower light sources of flexible shadowless light that can be adjusted arbitrarily, 5W high-power 275nm+395nm dual-wavelength ultraviolet light (used for cavity disinfection, ultraviolet mutagenesis) to form a clear floating dark field colony imaging effect d The uniform and shadowless lower light source is illuminated to highlight the colony morphology and realize the fool-style intelligent identification and counting e Upper light source, lower light source, upper and lower dual light sources, and ultraviolet light sources can be freely combined and switched independently to obtain the best colony imaging f Adapted to petri dish diameter: 50～130mm petri dish (pouring, membrane filtration, 3M paper) 2. Colony count analysis a. Automatic identification and statistics: SmartdownTM colony intelligent identification technology, with the feature of automatic enhancement of colony targets, can automatically identify large-scale clumps and chain-like adhesions of long-form bacilli (microscopic large-scale adhesions of Escherichia coli and pathogenic spores) and each on the plate. Class colonies (including Staphylococcus aureus count (GB4789.10-2016), coliform group determination (GB4789.3-2016), etc.). It can automatically form a batch processing wizard to realize one-click automatic counting b. The automatic counting accuracy is ≥96.5%, and the maximum monitoring correction is 3.5%, which is 100% correct. Analysis and statistics speed: 150～1000 colonies/s, the total time for plate imaging + counting ≤ 4 seconds c Colony adhesion segmentation: Automatically segment the long, round and other colony targets that adhere to each other into pieces, and the user can choose to segment or not to segment. It can also manually segment and merge colony target images d Color and shape recognition: With fast level set extraction technology, it can classify and count the number of colonies of specific colors and shapes according to 20 categories, and can convert and modify categories. 6 target areas can be selected at the same time for automatic analysis separately, and the analysis target areas can be added or edited freely e Colony morphology analysis: automatically obtain the area of each colony, equivalent diameter, major axis, minor axis, aspect ratio, roundness, perimeter, shape factor, etc. Filter and query by morphological indicators. f Mouse click correction, seamless removal of grids and text, automatic removal of impurities, effective support for complex microbial statistics, and automatic conversion of parameters in accordance with GB4789.2-2016. 3. Measurement of inhibition zone and analysis of antibiotic potency a One-key automatic determination of all inhibition zones in 6 90mm diameter plates at the same time, automatic determination of local adhesion inhibition zones, and automatic detection of the area and diameter of the inhibition zone in the presence of local text interference result. The measurement and analysis takes 1 to 4 seconds, and all kinds of analysis data can be saved or viewed in a unified manner. b The plate can be placed in any direction. It has the functions of automatic selection of bacteriostatic zone samples, fidelity visual comparison and editing. The reading resolution of the inhibition zone measurement system is ≤0.001mm, the highest optical measurement resolution is ≤0.0053mm, the repeated measurement error is ≤±0.01mm or ≤±0.1%; the potency measurement error is ≤±0.1%; the potency repeated measurement error is ≤ ±0.1%; measurement difference between stations ≤0.2% c Complies with the Chinese Pharmacopoeia, USP United States Pharmacopoeia, EP European Pharmacopoeia, and can complete the latest National Pharmacopoeia (2020 edition) one, two, and three doses and combined calculation, as well as antibiotic potency analysis of all strains. Combination optimization analysis feature with potency. 4. Export query and upgrade: The analysis image and data results can be saved, and the results can be reported directly without manual calculation. The results can be directly exported to Excel or PDF file reports. With online upgrade feature 5. Data tracing and rights protection: The system administrator can assign more than 10 operators to their respective usage rights, and the operation records can be traced back, which meets the requirements of GLP and GMP. 6. Measurement function: It can also measure the diameter of the inhibition zone (measurement resolution is 0.02mm), and can be automatically calibrated or manually calibrated. Can be dragged with the mouse to accurately measure length, angle, radian, area, arc, and arbitrary curve
1. Imaging device a It is equipped with a solid-state hard drive computer and an autofocus large depth of field 8 million pixels (3264x2448 pixels) color shooting light box capable of macro shooting. The highest resolution is 0.02mm, which can identify colonies as small as 0.05mm. With A4 format color CCD scanning imager (adjustable brightness, auto focus), it can realize floating dark-field imaging with an optical resolution of 40 million pixels b Fully enclosed dark box to eliminate environmental stray light interference c The upper and lower light sources of flexible shadowless light that can be adjusted arbitrarily, 5W high-power 275nm+395nm dual-wavelength ultraviolet light (used for cavity disinfection, ultraviolet mutagenesis) to form a clear floating dark field colony imaging effect d The uniform and shadowless lower light source is illuminated to highlight the colony morphology and realize the fool-style intelligent identification and counting e Upper light source, lower light source, upper and lower dual light sources, and ultraviolet light sources can be freely combined and switched independently to obtain the best colony imaging f Adapted to petri dish diameter: 50～130mm petri dish (pouring, membrane filtration, 3M paper)
