GV 6000M multi-function image workstation Introduction
1. Used in various chemiluminescence imaging such as ECL, ECLPlus, SuperSignal, etc.
2. For multicolor fluorescence imaging, such as Cy2, Cy3, Cy5, GFP, SYBR Green, SYBR Gold, SYBR Safe, FITC, GelStar, Fluorescein, Texas Red, fluorescent plates, etc.
3. DNA/RNA, protein gel electrophoresis imaging for dyeing various dyes;
4. For imaging of various hybrid membranes such as Western, Norther, Southern, Dot/Slot blot;
5. Other applications, such as petri dish colony count, ELISA plate, autoradiography film analysis, etc.
Feature
1. For imaging of chemiluminescence, multicolor fluorescence, gel, etc.;
2. High resolution CCD: using 6 megapixel CCD, with high resolution and sensitivity;
3. Deep cooling CCD: deep cooling CCD (up to 55 °C at ambient temperature), can perform long-term luminescence imaging;
4. Cooling speed is fast: it can be reduced to -20 °C in 3min;
5. Sample platform: double-layer adjustable stage to meet different experimental needs;
6. Pixel merging: further enhance the sensitivity of ECL imaging and capture weak illuminating signals by pixel merging (up to 24×24);
7. Super large aperture fixed focus lens: Using F0.95 large aperture electric fixed focus lens, with auto focus function, can automatically focus on the sample taken without manual adjustment;
8. Up to five kinds of fluorescent excitation light, which can meet various fluorescence experiments including multi-color fluorescent WB experiments.
Technical Parameters
1. High sensitivity cold CCD camera
1.1 Gray value: true 16bit
1.2 pixel value: 2688 × 2200, about 6 million pixels
1.3 pixel size: 4.54μm × 4.54μm
1.4 Dynamic Range: > 4.8 OD
1.5 Sensitivity: as low as pg grade DNA or fg grade protein
1.6 Refrigeration temperature: 55 ° C at ambient temperature
1.7 Refrigeration speed: It can be cooled to absolute temperature -20 °C (ambient temperature 25 °C) within 3 minutes, ready to use
1.8 Dark current: 0.00017e-/pixel/s
1.9 Binning: 1x1, 2x2, 4x4, 6x6, 8x8, 16x16, 24x24
1.10 CCD hardware dark current correction technology and pixel point defect correction technology greatly improve the CCD detection signal to noise ratio and obtain the best quality image.
1.11 QE: 75% @600nm
1.12 High quality f0.95/25mm wide-angle fixed-focus lens, large aperture, fast exposure
1.13 four exposure modes are available (Single Exposure, Multiple Exposure, TimeLapse Exposure, Progressive Exposure), exposure time 0.05sec-100min,
2. Black box and light source
2.1 Fully automatic 6-hole filter wheel, standard 535nm, 600nm, 700nm, 750nm, 830nm five emission filters, one-button automatic switching, to meet a variety of fluorescent and near-infrared two-color fluorescence imaging applications
2.2 Patented wide-angle emission filter technology, the perfect combination of two-phase color mirror and broadband pass filter, effectively remove stray light and improve the sensitivity of fluorescence detection.
2.3 Transmitted UV and illuminated white LED light source for conventional nucleic acid protein imaging applications
2.4 Five-color LED fluorescent light source (blue 475nm, green 530nm, red 620nm, near-infrared 685nm, infrared 760nm), energy 0-100% adjustable, to meet a variety of fluorescent and near-infrared two-color fluorescence imaging applications
3. Software
3.1 BioLight software includes image acquisition, enhancement and analysis functions, such as "Flat field correction" and "illumination correction" to optimize the performance and quantitative analysis capabilities of the Gel View system. The result is more accurate.
3.2 The software can perform qualitative and quantitative analysis, annotation, output image and other operations on different samples, such as strips, spots, bacterial clones, chips, cells or living animals.
3.3 Features of the pilot structure The operational process aids make the software easy to use.
3.4 Three-step lane and strip analysis can quickly calculate the molecular weight and quality of proteins and nucleic acids.
3.5 Image files are easy to transfer and share on a LAN or global network.
3.6 Western Marker and destination strips can be displayed simultaneously on one image
3.7 Analysis of relative quantification (ratio) and absolute quantification (concentration) of western results
3.8 Relative and absolute quantitative analysis of the microplate western
3.9 Multi-color fluorescence imaging function, the image of each fluorescent channel can be observed separately, and the fluorescence image can be synthesized and analyzed in any channel.
3.10 Total protein quantification normalization function, using internal reference protein for correction, reducing errors.
Introduction
1. Used in various chemiluminescence imaging such as ECL, ECLPlus, SuperSignal, etc.
2. For multicolor fluorescence imaging, such as Cy2, Cy3, Cy5, GFP, SYBR Green, SYBR Gold, SYBR Safe, FITC, GelStar, Fluorescein, Texas Red, fluorescent plates, etc.
3. DNA/RNA, protein gel electrophoresis imaging for dyeing various dyes;
4. For imaging of various hybrid membranes such as Western, Norther, Southern, Dot/Slot blot;
5. Other applications, such as petri dish colony count, ELISA plate, autoradiography film analysis, etc.
