Summary: Chemiluminescence (CL) is a rapidly developing non-radioimmunoassay technique in the last two decades. Chemiluminescence immunoassay (Chemiluminent Immunoassay CLIAA) is a newly developed immunoassay technique after enzyme immunoassay (EIA), radioimmunoassay (RIA) and fluorescence immunoassay (FIA). Because of its high specificity and small interference, the chemiluminescent immunoassay (CLIAA) technique has been developed by using the specificity of immune reaction and the signal specificity of chemiluminescence itself, just like RIA FIA and so on. Working principle: Chemiluminescence immunoanalyzer (CLIA) is a medical instrument for human immunoassay by detecting patient's serum. The quantitative patient serum and horseradish peroxidase (HRP) were added to the white opaque microporous plate coated with antibodies. The molecules to be tested in the serum were specifically bound to the conjugate of horseradish peroxidase and the antibody on the solid phase carrier. Separation and washing of unreacted free components. Then, the luminol Luminol luminescent substrate was added, and the intermediate was excited by the free energy released by the chemical reaction, and the energy was released in the form of photons from the ground state to the excited state. At this time, the microporous plate is placed into the analyzer, and the number of photons of each hole is read out by photon counter through the three-dimensional transmission system inside the instrument. The molecular concentration in the sample was quantitatively analyzed according to the mathematical model established by the standard sample. Finally, print the data report to assist the clinical diagnosis. Technical advantages: 1) A highly sensitive radioimmunoassay with a detection limit of 10-12 g / ml and an enzyme immunoassay of 10-9 g / ml and chemiluminescence of 10-16 g / ml. 2) The detection time is shorter than 1 second for each sample. From adding sample to getting the analysis result, it can be completed in 30-60 minutes. 3) A wide linear range of 7 × 107 relative luminescent units can be detected. 4)The retention period of reagents is more than one year, which has reached the international level. 5) Safe and environmentally friendly chemiluminescence immunoassay does not contain harmful chemical components. Performance features: 1) the instrument reagents have high cost-performance ratio and complete range of reagents. 2) Advanced determination method: high sensitive chemiluminescence immunoassay technique. 3) High efficient integrated design: precise three dimensional transmission, low loss fiber transmission, low noise operation, avoiding human error to improve the test accuracy. 4) system fault self-diagnosis function. 5) comprehensive quantitative analysis, multiple mathematical models built in, calibration curve automatically selected by the instrument. 6) consummate data processing, convenient hospital intranet, remote diagnosis and maintenance.
Summary: Chemiluminescence (CL) is a rapidly developing non-radioimmunoassay technique in the last two decades. Chemiluminescence immunoassay (Chemiluminent Immunoassay CLIAA) is a newly developed immunoassay technique after enzyme immunoassay (EIA), radioimmunoassay (RIA) and fluorescence immunoassay (FIA). Because of its high specificity and small interference, the chemiluminescent immunoassay (CLIAA) technique has been developed by using the specificity of immune reaction and the signal specificity of chemiluminescence itself, just like RIA FIA and so on.
