Introduction: CRY-3B cell fusion instrument is suitable for cell hybridization, cell fusion, direct observation of cell stringing under the inverted microscope, fusion phenomenon, and can be used in microbiology, animal medicine, bioengineering and other aspects. Compared with the traditional chemical fusion method, it has the characteristics of no toxicity to cells, high fusion efficiency, and convenience. A variety of flat and pin electrodes, nickel wire electrodes are available. The system adopts high-performance 32-bit ARM main control chip, all digital design, LCD touch screen, beautiful appearance, convenient and reliable operation. It is an ideal tool for colleges and universities and scientific research institutions to conduct cell fusion teaching and research. Cell fusion instrument electrical principle: The machine mainly produces alternating (string) pulse voltage, DC (fusion) single pulse voltage. The high frequency alternating current pulse (30 kHz - 3000 kHz 48 vp-p) generated by the alternating (string) pulse signal generating circuit can polarize the protoplasts and rapidly arrange the beaded shape. The DC (fusion) single-pulse generating circuit can emit a DC high-voltage pulse (5US-5000US, 0-700V) with adjustable pulse width according to the set number of times, so that the electric field intensity between the fusion electrodes can reach up to 6000V/CM instantaneously. The cell electrofusion mainly uses a certain value of the alternating electric field of frequency and voltage to arrange the protoplasts between the parallel electrodes at a certain interval to be beaded. Reversible breakdown of the cell membrane is induced by a certain value of a high-voltage direct current pulse electric field, thereby inducing fusion of cells in contact with each other. Because the cells can be polarized to form a dipole under the action of an external electric field, the adjacent cells will be close to each other due to the attraction of static electricity, and the cells will be sequentially directed to the electric field strength due to the uneven electric field generated between the electrodes of the fusion chamber. The high electrode parts move and are arranged in a bead shape. This arrangement is reversible. After the electric field disappears, the cells will return to a random distribution. In the uneven electric field, the pressure distribution on the cell membrane is uneven, resulting in the appearance of the protoplast cell membrane. Partial reversible breakdown, forming micropores. It automatically closes after the electric field disappears, but if the electric field strength exceeds the critical value, it will cause irreversible breakdown. Features: 1, multi-function: both cell electrofusion and electroporation 2, flexibility: has a wide range of voltage and pulse time 3, fast and efficient: simple and rapid completion of cell alignment, fusion, fusion and post-processing, only a few seconds 4, new LCD touch operation 5, the parameter settings are simple and convenient Application areas: 1. Cell fusion (the fusion process can be observed under an inverted microscope) 2, nuclear transfer 3. Embryo operation 4, hybridoma production 5. Plant protoplast fusion 6, chicken embryo egg import 7, living gene / drug introduction Technical Parameters: AC electric field parameter Square wave pulse voltage: Peak-to-peak value 0-58V (0-±29V), continuously adjustable Square wave into a pulse frequency: 30KHz-3000KHz continuously adjustable Gate time: 10-1000UM1US incrementally decreasing, continuously adjustable DC electric field parameters Square wave fusion pulse voltage: 5-600V continuously adjustable, the field strength can reach 6000V/cm when the electrode spacing is 1.0mm Square wave fusion pulse amplitude: 5-5000US, 1US incrementally decreasing, continuously adjustable Fusion (square wave) pulse 1-9 Random plate electrode: pole distance 0.5mm, 1mm, 2mm Display mode: 7 inch TFT touch screen display
Introduction: CRY-3B cell fusion instrument is suitable for cell hybridization, cell fusion, direct observation of cell stringing under the inverted microscope, fusion phenomenon, and can be used in microbiology, animal medicine, bioengineering and other aspects. Compared with the traditional chemical fusion method, it has the characteristics of no toxicity to cells, high fusion efficiency, and convenience. A variety of flat and pin electrodes, nickel wire electrodes are available. The system adopts high-performance 32-bit ARM main control chip, all digital design, LCD touch screen, beautiful appearance, convenient and reliable operation. It is an ideal tool for colleges and universities and scientific research institutions to conduct cell fusion teaching and research. Cell fusion instrument electrical principle: The machine mainly produces alternating (string) pulse voltage, DC (fusion) single pulse voltage. The high frequency alternating current pulse (30 kHz - 3000 kHz 48 vp-p) generated by the alternating (string) pulse signal generating circuit can polarize the protoplasts and rapidly arrange the beaded shape. The DC (fusion) single-pulse generating circuit can emit a DC high-voltage pulse (5US-5000US, 0-700V) with adjustable pulse width according to the set number of times, so that the electric field intensity between the fusion electrodes can reach up to 6000V/CM instantaneously. The cell electrofusion mainly uses a certain value of the alternating electric field of frequency and voltage to arrange the protoplasts between the parallel electrodes at a certain interval to be beaded. Reversible breakdown of the cell membrane is induced by a certain value of a high-voltage direct current pulse electric field, thereby inducing fusion of cells in contact with each other. Because the cells can be polarized to form a dipole under the action of an external electric field, the adjacent cells will be close to each other due to the attraction of static electricity, and the cells will be sequentially directed to the electric field strength due to the uneven electric field generated between the electrodes of the fusion chamber. The high electrode parts move and are arranged in a bead shape. This arrangement is reversible. After the electric field disappears, the cells will return to a random distribution. In the uneven electric field, the pressure distribution on the cell membrane is uneven, resulting in the appearance of the protoplast cell membrane. Partial reversible breakdown, forming micropores. It automatically closes after the electric field disappears, but if the electric field strength exceeds the critical value, it will cause irreversible breakdown.
