Introduction : ClearFirst - 5000Process type protein purification system is a key multi - channel parallel protein purification system developed by Shanghai flash spectrum biology , which is mainly used for large quantity of protein purification work in large - scale life sciences laboratories , which greatly improves the efficiency of purification work and saves a large amount of manpower and material resources compared with the traditional method . ClearFirst - 5000 Process - type protein purification system has unique advantages in pump , detector , fraction collector and control workstation software . It is a completely modularized product , which can separate five samples completely different . It can increase the number of channels flexibly , has extremely powerful function and extremely high cost performance , and is the ideal choice for high flux analysis and separation of protein purification laboratory in China . Instrument characteristics: 1 , A high - precision constant - current pump is used to form a high - precision gradient curve , so that the generation of bubbles is avoided , and the high - precision constant - current pump has extremely high practical value ; 2, The high - precision constant - current pump is used , the pulsation is small , the baseline is stable , the flow output is accurate , and the performance is much higher than that of the peristaltic pump system ; 3, The flow rate is 0 - 20 ml / min , the flow rate is 0 - 40 ml / min , and the analysis and preparation can be taken into account ; 4, Adopting a full - wavelength detector , and selecting an arbitrary wavelength between 190 - 850 nm for detection ; 5,The full - automatic fraction collector eliminates the phenomena of pipe skip , disturbance and overflow , and has high stability ;
6 ,The bottom of the X - Y matrix collecting device has no motor , and the safety is better than that of the disk type fraction collector ; 7, Collecting the fraction by using a 24 - hole or 96 - hole deep - hole micro - hole plate to save the collection volume ; 8, The high - precision fraction collection software ensures that the collection tube corresponds exactly to the peak position and convenient to find ; 9 ,The three - way valve with waste liquid collection is adopted to adapt to the continuous collection of high school pressure , so that only the required components can be collected ; 10,The peak collection judgment is accurate , omission and cross contamination are avoided , and manpower is saved ; 11, It has many modes such as peak collection , time , level , manual collection and so on , with high flexibility ; 12,The flow cell can adopt different optical paths to adapt to different analysis and purification requirements ; 13, A method database is provided , and different purification methods can be preset at any time to complete automatic operation ; 14 . A large number of special conductivity detectors with high precision and high precision are the same as those of imported products ; 15 . The standard PH detector has the function of temperature correction , the precision is better than the imported product ; 16 , the volume is compact , and the device is very convenient to put the equipment into a chromatographic refrigerator ; 17, The pressure - bearing pipeline of the system is basically PEEK material , and the pressure resistance and biocompatibility are good ; 18 ,The sampling mode of the multi - way valve is used , the sample injection is accurate , and air is prevented from entering the system ; 19,The instrument can be fully controlled by computer , which greatly reduces the use difficulty ; 20, Instrument self - inspection , user management and sample management can be carried out , and the information system can be connected ; 21,Five channels can be used for parallel separation , the separation flux is large , and the method is suitable for a large quantity of samples ; 22,The wavelength accuracy is better than 0.2 nm , and the detection sensitivity is better than that of diode array type products . 23, All Chinese interfaces of the workstation are convenient to use and conform to the habits of Chinese people ; 24, Only upgrade the multi - pass valve buffer expansion assembly ( 8 or 12 ) to form more types of buffers ; 25,The instrument is completely modularized , saves space , is easy to increase and decrease , and has high flexibility ; 26,The instrument can be equipped with a large volume injection system and a sampling pump for large volume injections ;
27,The instrument can use a multi - channel chromatographic column switching system for different chromatographic column switching ; 28,A chromatographic column with different fillers can be used for each channel , and different separation systems can be used ; 29,Different injection rings can be used for each channel , and the sampling amount can be completely different ; 30,Different detection wavelengths can be used for each channel to correspond to different samples ; Instrument Composition : 1 . Host ( including high precision constant current pump , buffer selection valve , multi - pass sampling valve ) ; 2 . Detector ( including full - wavelength ultraviolet detector , conductivity detector , digital - to - analog converter ) ; 3 . Fully automatic fraction collector ; 4 . Special workstation for purification of CStar - 5000 protein ; Instrument parameters : 1 . Solvent compatibility : the aqueous buffer used for the purification of commonly used biomolecules ; 2 . Flow rate range : 0 - 20ml / min ( optional 0 - 40ml / min ) ; 3 . Pressure range : 0 - 0.7MPa ( 7 bar , 100 psi ) 4 . Viscosity : up to 5 cP ; 5 . Wavelength range : 190 - 850 nm ; 6 . Optical path length : the standard is 2mm , the rest can be customized ; 7 . Conductivity range : 1uS / cm - 999.9mS / cm ; 8 . PH determination range : 0 - 14.00 ; 9 . PH determination accuracy : 0.01N unit , temperature compensation ; 10 . Channel number : 5 channels ; Purpose of the instrument : 1 . Protein purification ( gel , ion exchange , hydrophobic interaction , affinity , etc . ) ; 2 , Separating and purifying the nucleic acid ; 3 , Separating and purifying antibiotic polymer impurities ; 4 , Separating and purifying the natural product ;
Introduction : ClearFirst - 5000Process type protein purification system is a key multi - channel parallel protein purification system developed by Shanghai flash spectrum biology , which is mainly used for large quantity of protein purification work in large - scale life sciences laboratories , which greatly improves the efficiency of purification work and saves a large amount of manpower and material resources compared with the traditional method . ClearFirst - 5000 Process - type protein purification system has unique advantages in pump , detector , fraction collector and control workstation software . It is a completely modularized product , which can separate five samples completely different . It can increase the number of channels flexibly , has extremely powerful function and extremely high cost performance , and is the ideal choice for high flux analysis and separation of protein purification laboratory in China .
